Sequencing the Blood Brain
Barrier in Alzheimer’s Disease

Gabriel Mateus Bernardo Harrington

Research Associate | Webber Group

2024-03-14

Project design

  • Sequencing post-mortem human pre-frontal cortex
  • 20 cases and 20 controls
  • Sample prep that separates the vascular fraction and the parenchymal fraction of the tissue
    • So 2 per donor - 80 samples sequenced
  • 10X seq
  • Processed data with scFlow

A cartoon deliniating the parenchyma and vascular components of the brain

UMAP

UMAP plot coloured by manual cell type annotations. Similarly to the prior UMAP plots, the vascular cell types are in the large cluster to the right, and the neuronal cells are in the other clusters.

MAGMA

Thanks for listening

References

Made with the UKDRI Quarto template by GMBH. Code available on GitHub here. Licensed under CC-BY-SA

Warren, Katherine Elizabeth. 2018. “Beyond the Blood:brain Barrier: The Importance of Central Nervous System (CNS) Pharmacokinetics for the Treatment of CNS Tumors, Including Diffuse Intrinsic Pontine Glioma.” Frontiers in Oncology 8. https://www.frontiersin.org/articles/10.3389/fonc.2018.00239.